Biomimetics and Encapsulation Research Technology Lab

Our research fields

Our research is focused on the development of nanomedicines to treat inflammatory diseases (cardiovascular, neurological disease and infectious disease), with large emphasis on in vivo pre-clinical studies. In collaborations with clinicians from different hospitals or institutes in Singapore, we developed a highly translational research, and is broadly applicable to many pathological conditions. The lab specifically developed an expertise on extracellular vesicles as exosomes mimetics in eukaryote and prokaryote cells for therapeutic drug delivery or vaccines.

  • Nanomedicine & Drug delivery with nanoparticles.
  • Cells-derived nanoparticles as exosome mimetics.
  • Bacterial extracellular vesicles for vaccine and antibiotics resistance.
  • Biodistribution of nanoparticles.
Drug delivery systems

Drug delivery systems

Vaccin and antibiotics resistance

Vaccin and antibiotics resistance

Extracellular vesicles mimetics

Extracellular vesicles mimetics

Bert Lab Team’s

LAB Head

Bertrand Czarny

Research fellow

Krishna Radhakrishnan

PHD Students

Syed Abdullah

shaun_ok

Shaun Lim Wen Zheng

Pei Qi

Lim Pei Qi

Melvin Liew

Staff

Anu Maashaa

Anu Maashaa

Publications

  1. Neupane Y.R., Huang C., Wang X., Chng W.H., Venkatesan G., Zharkova O., Wacker M.G., Czarny B., Storm G., Wang J.-W., and Pastorin G. (2021). Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles. Pharmaceutics, 13(7), 1052. READ
  2. Ou Y.-H., Shui Z., Wang J.W., Czarny B., and Pastorin G. (2021). Cell-Derived Nanovesicles As Exosonne-Mimetics For Drug Delivery Purposes: Uses & Recommendations. In: Narayanan K. (eds) Bio-Carrier Vectors: Methods and Protocols, vol 2211. New York: Springer. ISBN: 978-1-0716-0942-2. pp. 147-170. READ
  3. Ou Y.-H., Liang J., Czarny B., Wacker M.G., Yu V., Wang J.-W., and Pastorin G. (2021). Extracellular Vesicle (EV) biohybrid systems for cancer therapy: Recent advances and future perspectives. Seminars in Cancer Biology. READ

More publications

Articles
  1. Neupane Y.R., Huang C., Wang X., Chng W.H., Venkatesan G., Zharkova O., Wacker M.G., Czarny B., Storm G., Wang J.-W., and Pastorin G. (2021). Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles. Pharmaceutics, 13(7), 1052. READ
  2. Ou Y.-H., Shui Z., Wang J.W., Czarny B., and Pastorin G. (2021). Cell-Derived Nanovesicles As Exosonne-Mimetics For Drug Delivery Purposes: Uses & Recommendations. In: Narayanan K. (eds) Bio-Carrier Vectors: Methods and Protocols, vol 2211. New York: Springer. ISBN: 978-1-0716-0942-2. pp. 147-170. READ
  3. Ou Y.-H., Liang J., Czarny B., Wacker M.G., Yu V., Wang J.-W., and Pastorin G. (2021). Extracellular Vesicle (EV) biohybrid systems for cancer therapy: Recent advances and future perspectives. Seminars in Cancer Biology. READ
  4. Huang C., Neupane Y.R., Lim X.C., Shekhani R., Czarny B., Wacker M.G., Pastorin G., and Wang J.-W. (2020). Extracellular vesicles in cardiovascular disease. In: Makowski G.S. (eds) Advances in Clinical Chemistry, Elsevier, 2020. READ
  5. Vidaillac C., Yong C.F.L., Aschtgen M.-S., Qu J., Yang S., Xu G., Seng Z.J., Brown A.C., Ali M.K., Jaggi T.K., Sankaran J., Foo Y.H., Righetti F., Nedumaran A.M., Mac Aogáin M., Roizman D., Richard J.-A., Rogers T.R., Toyofuku M., Luo D., Loh E., Wohland T., Czarny B., Horvat J.C., Hansbro P.M., Yang L., Li L., Normark S., Henriques-Normark B., and Chotirmall S.H. (2020). Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa. mBio, 11(5), e01774-20. READ
  6. Ou Y.-H., Zou S., Goh W.J., Chong S.Y., Venkatesan G., Wacker M.G., Storm G., Wang J.-W., Czarny B., Pastorin G., and Woon C.Y. (2020).Micro Cell Vesicle Technology (mCVT): A Novel Hybrid System of Gene Delivery for Hard-To-Transfect (HTT) Cells. Nanoscale, 12, 18022-18030. READ
  7. Darwitan A., Wong Y.S., Nguyen L.T.H.,Czarny B., Vincent A., Nedumaran A.M., Tan Y.F., Muktabar A., Tang J.K., Ng K.W. and Venkatraman S.S. (2020). Liposomal Nanotherapy for Treatment of Atherosclerosis. Advanced Healthcare Materials, 9(14), 2000465. READ
  8. Lim SWZ, Wong YS, Czarny B, Venkatraman SS. Microfluidic-directed self-assembly of liposomes: Role of interdigitation. Journal of Colloid and Interface Science, Jun 2020, IP 6.3. READ
  9. Darwitan A, Tan YF, Wong YS, Nedumaran AM, Czarny B, Venkatraman SS. Targeting efficiency of nanoliposomes on atherosclerotic foam cells: Polyethylene glycol-to-ligand ratio effects Expert Opinion on Drug Delivery Jun 2020, IP 3.5. READ
  10. SA Alkaff, K Radhakrishnan, AM Nedumaran, P Liao, B Czarny. Nanocarriers for Stroke Therapy: Advances and Obstacles in Translating Animal Studies. International Journal of Nanomedicine. Jan 2020, IP 5.0. READ
  11. Saw S, Aiken A, Fang H, McKee TD, Bregant S, Sanchez O, Chen Y, Weiss A, Dickson BC, Czarny B, Sinha A, Fosang A, Dive V, Waterhouse PD, Kislinger T, Khokha R. Metalloprotease inhibitor TIMP proteins control FGF-2 bioavailability and regulate skeletal growth..J Cell Biol. 2019 Aug, IP 8.9. READ
  12. Babak MV, Zhi Y, Czarny B, Toh TB, Hooi L, Chow EK, Ang WH, Gibson D and Pastorin G. Dual-targeting Dual-action Platinum(IV) Platform for Enhanced Anticancer Activity and Reduced Nephrotoxicity Angewandte Chemie, April 2019, IP 12.3. READ
  13. Wong YS, Czarny B, Venkatramana SS. Precision nanomedicine in atherosclerosis therapy: how far are we from reality? Precision Nanomedicine, Jan 2019. READ
  14. Wong CW*, Czarny B*, Metselaar J, Ho C, Ng SR, Veluchamy AB, Storm G, and Wong T Evaluation of subconjunctival liposomal steroids for the treatment of experimental uveitis Scientific report April 2018, IP 4.1. READ
  15. Goh WJ, Zou S, Czarny B, Pastorin G. nCVTs: A Hybrid Smart Tumour Targeting Platform, Mar 2018, IP 7.2. READ
  16. Obuobi S, Voo ZX, Low MW, Czarny B, Selvarajan V, Ibrahim NL, Yang YY and Ee PLR. Phenylboronic Acid Functionalized Polycarbonate Hydrogels for Controlled Release of Polymyxin B in Pseudomonas Aeruginosa Infected Burn Wounds, Advanced Healthcare Materials. Mar 2018, IP 6.3. READ
  17. Goh WJ, Zou S, Lee CK, Wang JW, Czarny B, Pastorin G. EXOPLEXs: Chimeric Drug Delivery Platform from the fusion of Cell Derived Nanovesicles and Liposomes biomacromolecules. Nov 2017, IP 5.7. READ
  18. Goh WJ, Ong WY, Torta F, Schiffelers RM, Storm G, Pastorin G *, Czarny B*. Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: A Cost-Effective Alternative, Scientific report. Oct 2017, IP 4.1. READ
  19. Nayak TR, Hao W, Pant A, Zheng M, Junginger H, Goh WJ, Lee CK, Zou S, Alonso S, Czarny B, Storm G, Sow CH, Lee C and Pastorin G. ZnO nano-rod devices for transdermal delivery and immunization, Nanomaterials, June 2017, IP 3.5. READ
  20. Goh WJ, Lee CK, Zou S, Woon E, Czarny B, Pastorin G. Doxorubicin loaded Cell Derived Nanovesicles: An Alternative Targeted Approach for Anti-Tumor Therapy, International Journal of Nanomedicine, February 2017, IP 4.4. READ
  21. Czarny BM, Allijn IE, Wang X, Chong SY, Weiler M, da Silva AE, Metselaar JM, Lam CS, Pastorin G, de Kleijn DP, Storm G, Wang JW, Schiffelers RM. Liposome encapsulated berberine treatment attenuates cardiac dysfunction after myocardial infarction. J Control Release. January 2017, IP 7.9. READ
  22. Rouanet-Mehouas C, Czarny B, Beau F, Cassar-Lajeunesse E, Stura EA, Dive V, Devel L. Zinc-Metalloproteinase Inhibitors: Evaluation of the Complex Role Played by the Zinc-Binding Group on Potency and Selectivity. J Med Chem. 2016, IP 5.4. READ
  23. Audisio, D; Czarny, B; Georgin, D; Berthon, F; Plastow, G; Pinault, M; Patriarche, G; Thuleau, A; L’Hermite, MM; Dive, V; Tarana, F. Combining efficient C-14-radiolabeling and radioimaging techniques of manufactured nanoparticles for toxicological studies J Labelled Comp Radiopharm. 2016, IP 1.4. READ
  24. Li J, Yoong L, Goh WJ, Czarny B, Yang Z, Poddar K, Dykas M, Patra A, Venkatesan T, Lee C and Pastorin G; In vitro Controlled Release of Cisplatin from Gold-Carbon Nanobottles via Cleavable Linkages, International Journal of Nanomedicine, 2015, IP 4.4. READ
  25. Pant K, Gröger D, Bergmann R, Pietzsch J, Steinbach J, Graham B, Spiccia L, Berthon F, Czarny B, Devel L, Dive V, Stephan H, Haag R. Synthesis and Biodistribution Studies of 3H- and 64Cu-labeled Dendritic Polyglycerol and Dendritic polyglycerol Sulfate. Bioconjugate Chem., April 19, 2015, IP 4.5. READ
  26. Czarny B., Georgin D., Berthon F., Plastow G., Pinault M., Patriarche G., Thuleau A., Mayne-L’Hermite M., Taran F. and Dive V., Carbon nanotube translocation to distant organs after pulmonary exposition: insights from in situ 14C radiolabelling and tissue radioimaging. ACS Nano, 2014 May 28, IP 13.7. READ
  27. Meides A ., Gutschalk CM., Devel L., Beau F., Czarny B., Hensler S., Neugebauer J., Dive V., Angel P., Mueller MM., Effects of selective MMP-13 inhibition in Squamous Cell Carcinoma depend on estrogen. Int J Cancer, 2014 Mar 27, IP 7.4. READ
  28. Tranchant I.,Vera L., Czarny B., Amoura M., Cassar-Lajeunesse E., Beau F., Stura E.A., and Dive V., Halogen Bonding Controls Selectivity of FRET Substrate Probes for MMP-9. Chemistry and biology, 2014 Mar 20, IP 7.6. READ
  29. Masuyer G., Akif M., Czarny B., Beau F., Schwager S., Sturrock E.D., Isaac R.E., Dive V. and Acharya R., Crystal structures of highly specific phosphinic tripeptide enantiomers in complex with the angiotensin-I converting enzyme. FEBS, 2014 Feb, IP 4.5. READ
  30. Georgin D., Czarny B., Ferrie M., Pinault M., Mayne-L’Hermite M., Carriere M., Dive V. and Taran F., On the trail of in vivo carbon nanotubes, 2013 Biofutur 347: p. 25-28 IP 0.1. READ
  31. Czarny B., Georgin D., Pinault M. and Dive V. Carbon nanotubes passes pulmonary barrier 2013 Biofutur 358: p. 52-53, IP 0.1. READ
  32. Antoni C., Vera L., Devel L., Catalani M.P., Czarny B., Cassar-Lajeunesse E., Nuti E., Rossello A., Dive V., and Stura E.A., Crystallization of bi-functional ligand protein complexes. J Struct Biol, 2013. 182(3): p. 246-54. IP 3.4. READ
  33. Vera L., Antoni C., Devel L., Czarny B., Cassar-Lajeunesse E., Rossello A., Dive V., and Stura E.A., Screening Using Polymorphs for the Crystallization of Protein−2 Ligand Complexes Crystal Growth & Design, 2013. 13(5):  1878-1888, IP 4.0. READ
  34. Czarny B., Stura E.A., Devel L., Vera L., Cassar-Lajeunesse E., Beau F., Calderone V., Fragai M., Luchinat C., and Dive V., Molecular determinants of a selective matrix metalloprotease-12 inhibitor: insights from crystallography and thermodynamic studies. Journal of Medicinal Chemistry, 2013. 56(3): p. 1149-59. IP 6.3. READ
  35. Nury C., Bregant S., Czarny B., Berthon F., Cassar-Lajeunesse E., and Dive V., Detection of Endogenous Matrix Metalloprotease-12 Active Form with a Novel Broad Spectrum Activity-based Probe. Journal of biological chemistry, 2013. 288(8): p. 5636-44. IP 4.0. READ
  36. Nury C., Czarny B., Cassar-Lajeunesse E., Georgiadis D., Bregant S., and Dive V., A Pan Photoaffinity Probe for Detecting Active Forms of Matrix Metalloproteinases. ChemBioChem, 2013. 14(1): p. 107-114. IP 2.8. READ
  37. Larue C., Pinault M., Czarny B., Georgin D., Jaillard D., Bendiab N., Mayne-L’Hermite M., Taran F., Dive V., and Carrière M., Quantitative evaluation of multi-walled carbon nanotube uptake in wheat and rapeseed. Journal of Hazardous Materials, 2012. 227–228(0): p. 155-163. IP 6.4. READ
  38. Devel L., Beau F., Amoura M., Vera L., Cassar-Lajeunesse E., Garcia S., Czarny B., Stura E.A., and Dive V., Simple Pseudo-dipeptides with a P2′ Glutamate: a novel inhibitor family of matrix metalloproteases and other metzincins. Journal of biological chemistry, 2012. 287(32): p. 26647-56. IP 4.0. READ
  39. Martinage O., Le Clainche L., Czarny B., and Dugave C., Synthesis and biological evaluation of a new triazole-oxotechnetium complex. Organic & Biomolecular Chemistry, 2012. 10(32): p. 6484-6490. IP 3.4. READ
  40. Garofalakis A., Dubois A., Thézé B., Czarny B., Tavitian B., and Ducongé F., Fusion of [[(18)F]FDG PET with Fluorescence Diffuse Optical Tomography to Improve Validation of Probes and Tumor Imaging. Molecular Imaging and Biology, 2012: p. 1-10. IP 3.4. READ
  41. Vera L., Czarny B., Georgiadis D., Dive V., and Stura E.A., Practical Use of Glycerol in Protein Crystallization. Crystal Growth & Design, 2011. 11(7): p. 2755-2762 IP 4.0. READ
  42. Akif M., Schwager S.L., Anthony C.S., Czarny B., Beau F., Dive V., Sturrock E.D., and Acharya K.R., Novel mechanism of inhibition of human angiotensin-I-converting enzyme (ACE) by a highly specific phosphinic tripeptide. Biochemical Journal, 2011. 436(1): p. 53-59. IP 3.9. READ
  43. Aufort M., Gonera M., Lelait M.-A., Czarny B., Le Clainche L., Thaï R., Landra A., Ruinart de Brimont M., and Dugave C., Synthesis, in vitro screening and in vivo evaluation of cyclic RGD analogs cyclized through oxorhenium and oxotechnetium coordination. European Journal of Medicinal Chemistry, 2011. 46(5): p. 1779-1788. IP 4.8. READ
  44. Aufort M., Gonera M., Le Gal J., Czarny B., Le Clainche L., Thai R., and Dugave C., Oxorhenium-Mediated Assembly of Noncyclic Selective Integrin Antagonists: A Combinatorial Approach. ChemBioChem, 2011. 12(4): p. 583-592. IP 2.8. READ
  45. Johnson J.L., Devel L., Czarny B., George S.J., Jackson C.L., Rogakos V., Beau F., Yiotakis A., Newby A.C., and Dive V., A Selective Matrix Metalloproteinase-12 Inhibitor Retards Atherosclerotic Plaque Development in Apolipoprotein E-Knockout Mice. Arteriosclerosis Thrombosis and Vascular Biology, 2011. 31(3): p. 528-U127. IP 6.1. READ
  46. Devel L., Garcia S., Czarny B., Beau F., Lajeunesse E., Vera L., Georgiadis D., Stura E., and Dive V., Insights from Selective Non-phosphinic Inhibitors of MMP-12 Tailored to Fit with an S-1 ‘ Loop Canonical Conformation. Journal of Biological Chemistry, 2010. 285(46): p. 35900-35909. IP 4.0. READ
  47. Devel L., Czarny B., Beau F., Georgiadis D., Stura E., and Dive V., Third generation of matrix metalloprotease inhibitors: Gain in selectivity by targeting the depth of the S ‘(1) cavity. Biochimie, 2010. 92(11): p. 1501-1508. IP 3.2. READ
  48. Pallafacchina G., François S., Regnault B., Czarny B., Dive V., Cumano A., Montarras D., and Buckingham M., An adult tissue-specific stem cell in its niche: A gene profiling analysis of in vivo quiescent and activated muscle satellite cells. Stem Cell Research, 2010. 4(2): p. 77-91. IP 1.8. READ
  49. Georgin D., Czarny B., Botquin M., Mayne-L’Hermite M., Pinault M., Bouchet-Fabre B., Carriere M., Poncy J.-L., Chau Q., Maximilien R., Dive V., and Taran F., Preparation of 14C-Labeled Multiwalled Carbon Nanotubes for Biodistribution Investigations. Journal of the American Chemical Society, 2009. 131(41): p. 14658-14659. IP 14.4. READ
  50. Dabert-Gay A.S., Czarny B., Lajeunesse E., Thai R., Nagase H., and Dive V., Covalent Modification of Matrix Metalloproteinases by a Photoaffinity Probe: Influence of Nucleophilicity and Flexibility of the Residue in Position 241. Bioconjugate Chemistry, 2009. 20(2): p. 367-375. IP 4.4. READ
  51. Bregant S., Huillet C., Devel L., Dabert-Gay A.-S., Beau F., Thai R., Czarny B., Yiotakis A., and Dive V., Detection of Matrix Metalloproteinase Active Forms in Complex Proteomes: Evaluation of Affinity versus Photoaffinity Capture. Journal of Proteome Research, 2009. 8(5): p. 2484-2494. IP 4.0. READ
  52. Dabert-Gay A.S., Czarny B., Devel L., Beau F., Lajeunesse E., Bregant S., Thai R., Yiotakis A., and Dive V., Molecular Determinants of Matrix Metalloproteinase-12 Covalent Modification by a Photoaffinity probe insights into activity- based probe development and conformational variability of matrix metalloproteinases. Journal of Biological Chemistry, 2008. 283(45): p. 31058-31067. IP 4.0. READ
  53. Georgiadis D., Beau F., Czarny B., Cotton J., Yiotakis A., and Dive V., Roles of the Two Active Sites of Somatic Angiotensin-Converting Enzyme in the Cleavage of Angiotensin I and Bradykinin. Circulation Research, 2003. 93(2): p. 148-154. IP 15.2. READ

Book chapter’s
  1. Cell-Derived Nanovesicles As Exosome-Mimetics For Drug Delivery Purposes: Uses & Recommendations, Ou YH, Shui Z, Wang JW, Czarny B, Pastorin G, “Bio-Carrier Vectors” in Methods in Molecular Biology series by Springer Nature, 2020 DOI:10.1007/978-1-0716-0943-9
  2. Extracellular vesicles in cardiovascular diseases, Huang C, Neupane YR, Lim CX, Shekhani R, Czarny B, Wacker MG, Pastorin G, Wang JW, Advances in Clinical Chemistry, 2020, DOI: 10.1016/bs.acc.2020.08.006
Patents
  • Patent Number: US 07541157 / WO2011/023864, 2011, CEA, Pseudo-dipeptides as MMP inhibitors, DEVEL Laurent ; DIVE Vincent ; BEAU Fabrice ; CZARNY Bertrand.

 

 

    • Patent application: 2017/3082562576U / C201703740Y, 2017, Enceladus Pharmaceuticals B.V. Universiteit Utrecht Holding B.V. Singapore Eye Research Institute. Liposomal corticosteroids for topical injection in inflamed lesions or areas.

Get in touch with us

    School of Materials Science and Engineering

    http://www.mse.ntu.edu.sg

    Nanyang Technological University
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    Singapore 639798